| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068925 |
| Chromosome: | chromosome 10 |
| Location: | 3968931 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448650 | KDG5 | Diacylglycerol kinase; (1 of 1) IPR005218 - Diacylglycerol/lipid kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTTAGTTTGGGCTGGTATTTAAACCCC |
| Internal bar code: | TTCAAGCGGAGGTTTGAAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCATATGAGGCAAAAG |
| Suggested primer 2: | TTCGCTGGGAATATTTGACC |