| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068946 |
| Chromosome: | chromosome 9 |
| Location: | 1241913 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399550 | (1 of 1) PTHR10857//PTHR10857:SF11 - COPINE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTCCTGCTCCGACTTCTGGTGGTCCAT |
| Internal bar code: | GGCACCCAAATAATGCGTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 99.8339 |
| LEAP-Seq n confirming: | 3006 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGTAACCCCCATCTCCT |
| Suggested primer 2: | GGTCAAGGAAGAGCATGAGC |