| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.068975 |
| Chromosome: | chromosome 2 |
| Location: | 1416920 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083450 | (1 of 1) IPR000104//IPR002938 - Antifreeze protein, type I // FAD-binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTGTGTGTCGCTGTGTGACTACGGTTT |
| Internal bar code: | GAGGATGGGGGGGGACCAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 344 |
| LEAP-Seq percent confirming: | 98.5955 |
| LEAP-Seq n confirming: | 1053 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTACCCACCCTATCCAC |
| Suggested primer 2: | TTCGCCAATGTGAGGTGTAA |