Insertion junction: LMJ.RY0402.069015_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.069015
Chromosome:chromosome 16
Location:395812
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g693601 ISG-C2,ISG2 (1 of 5) PTHR16631//PTHR16631:SF9 - FAMILY NOT NAMED // GLUCAN 1,3-BETA-GLUCOSIDASE; Hydroxyproline-rich cell wall protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCACGGGGGTGCATTTATCTGATACAGG
Internal bar code:TATTTGGAAGATAAAATCTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:548
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:608
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTCCTTCTCCCTCTCCT
Suggested primer 2:CAGGATTGACACGCTGCTAA