Insertion junction: LMJ.RY0402.069052_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.069052
Chromosome:chromosome 3
Location:5570865
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g186550 (1 of 1) K11137 - telomere length regulation protein (TELO2, TEL2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCTGCCTGAACCTGGTCGACCAACCTGC
Internal bar code:TCGTATAGTGCCACACGCTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:124
LEAP-Seq percent confirming:49.4954
LEAP-Seq n confirming:1128
LEAP-Seq n nonconfirming:1151
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGATGTGTGCGCTTGAG
Suggested primer 2:TGCCACCTACCCTACTCCAC