Insertion junction: LMJ.RY0402.069054_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.069054
Chromosome:chromosome 10
Location:2707530
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g438150 (1 of 2) IPR001841//IPR001876//IPR016024 - Zinc finger, RING-type // Zinc finger, RanBP2-type // Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCGAAGAACATCTTCAGGTGCGTGTCA
Internal bar code:TTGCGCGTCGCGTTTTATTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:448
LEAP-Seq percent confirming:99.6983
LEAP-Seq n confirming:12557
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCCACACATCCAGTTGTC
Suggested primer 2:CTAGGACAGCGAACAAAGGC