| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069102 |
| Chromosome: | chromosome 16 |
| Location: | 1203083 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650750 | FAP268 | (1 of 18) PTHR10891 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN; Flagellar Associated Protein 268 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAAACACGAAAGGAGCCCGTCTGTATGT |
| Internal bar code: | TGATGGGAGTTGTTTTCTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 522 |
| LEAP-Seq percent confirming: | 97.4026 |
| LEAP-Seq n confirming: | 825 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTCAATGCTGTGGTTCC |
| Suggested primer 2: | GTTCTGTGGGGAGTGACGAT |