| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069140 |
| Chromosome: | chromosome 1 |
| Location: | 5083610 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035300 | CPA2 | (1 of 2) 3.5.1.53 - N-carbamoylputrescine amidase; N-carbamoylputrescine amidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGTCGGTGCGGTAGTGGCCGAGCAGACC |
| Internal bar code: | GAGTCTCCGCTTGCCGGAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 99.5095 |
| LEAP-Seq n confirming: | 1623 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACATGACAACGCCAATAC |
| Suggested primer 2: | GCAAATGGGGAAGTCTTTGA |