Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.069152 |
Chromosome: | chromosome_17 |
Location: | 3219853 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g722350 | RABL2A | Small Rab-related GTPase | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCTTTGCCCGCCCGCCCAGGGGCGAGCACG |
Internal bar code: | GCATATATTTACATAGCCCTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 347 |
LEAP-Seq percent confirming: | 99.3968 |
LEAP-Seq n confirming: | 2307 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGAGTGCATGCTGTTGAAG |
Suggested primer 2: | AAAGTCCAACTCGCCTAGCA |