| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.069162 |
| Chromosome: | chromosome 3 |
| Location: | 2590602 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160550 | MTP4 | Metal Transport Protein 4; (1 of 3) PTHR11562:SF21 - PROTEIN R02F11.3, ISOFORM B | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTGAACCCGGGTTGCGTAAATGCGTCT |
| Internal bar code: | TGCGAAAAATCTCGAGCGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 325 |
| LEAP-Seq percent confirming: | 83.1615 |
| LEAP-Seq n confirming: | 1936 |
| LEAP-Seq n nonconfirming: | 392 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTGTCTCTCTATCCGGC |
| Suggested primer 2: | ATCAGAGCCAGTGGCAGACT |