| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069168 |
| Chromosome: | chromosome_4 |
| Location: | 1896776 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g218000 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGCACACCCAATGCACCCGCCCCCAGCCC |
| Internal bar code: | ACGCTGCCAGACGGGCCGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 65 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAGGTGCCGTGTATCCT |
| Suggested primer 2: | GCTGTTCCCAACGCTCTTAC |