| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069221 |
| Chromosome: | chromosome 17 |
| Location: | 3496277 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725100 | (1 of 1) PTHR11390:SF20 - DNA TOPOISOMERASE 3-BETA-1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCGAGCTGGCTGGCCACGGAAGCGCCC |
| Internal bar code: | CGCTCCTTCTCTTGCATACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 99.1813 |
| LEAP-Seq n confirming: | 848 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGGAGTTGAAATCGTGT |
| Suggested primer 2: | GCACATACGGCATACACGAC |