| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069221 |
| Chromosome: | chromosome 17 |
| Location: | 3496284 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725100 | (1 of 1) PTHR11390:SF20 - DNA TOPOISOMERASE 3-BETA-1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGGTGAGTGACCCCAGCACCACCACCG |
| Internal bar code: | TGTCAGTCGGCGTCGGCTCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 36.6578 |
| LEAP-Seq n confirming: | 3705 |
| LEAP-Seq n nonconfirming: | 6402 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGGAGTTGAAATCGTGT |
| Suggested primer 2: | GCACATACGGCATACACGAC |