| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069240 |
| Chromosome: | chromosome 2 |
| Location: | 2417170 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091450 | MRPS4,uS4m | Mitochondrial ribosomal protein S4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCAAGCTCTTGCTCAGCGCGCCGTTCCA |
| Internal bar code: | CTCTGTTTTATCTGGCATTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 250 |
| LEAP-Seq percent confirming: | 85.2359 |
| LEAP-Seq n confirming: | 2927 |
| LEAP-Seq n nonconfirming: | 507 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAACCCCACCCTACTTA |
| Suggested primer 2: | CATATACTGAGGGCCAGGGA |