Insertion junction: LMJ.RY0402.069240_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.069240
Chromosome:chromosome 3
Location:3622045
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g168650 (1 of 1) PF08621 - RPAP1-like, N-terminal (RPAP1_N) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATCCGCGCGACCGCACCCCATACATGCA
Internal bar code:TGACCGTGTTTCACGGCCCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:45
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCAGACAAGCAGGAGGG
Suggested primer 2:CAGGAAGCTTTGGTCTCAGG