Insertion junction: LMJ.RY0402.069264_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.069264
Chromosome:chromosome 10
Location:1145551
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g425700 NUP107 (1 of 1) K14301 - nuclear pore complex protein Nup107 (NUP107, NUP84); Nucleoporin 107 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGCACACGCACCTGCAGCTCTGGGTAG
Internal bar code:TGCGCAAAGGCCACGCCGGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:25
LEAP-Seq percent confirming:40.5904
LEAP-Seq n confirming:990
LEAP-Seq n nonconfirming:1449
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGGGAAGTTGCAAGATGG
Suggested primer 2:CCATGGGATGGTGTGTATCA