| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.069271 |
| Chromosome: | chromosome 7 |
| Location: | 4193730 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342250 | PFD2 | Prefoldin molecular chaperone, subunit 2; (1 of 1) K09549 - prefoldin subunit 2 (PFDN2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGCACCCTCTTCTTGCCGGGGTCGGCT |
| Internal bar code: | ATCTGTTCAGAGTCCTGTACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 99.8178 |
| LEAP-Seq n confirming: | 2191 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAACCGAAAAGGTGTGT |
| Suggested primer 2: | AAAGGACTGAGCAAGGCAAA |