| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069274 |
| Chromosome: | chromosome 6 |
| Location: | 268280 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g250850 | MSH1 | DNA mismatch repair protein, MutS homolog; (1 of 1) PTHR11361:SF82 - DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACGGGAAGGGGGGGTGCGCTTTGGGCT |
| Internal bar code: | CCTTTCGCTGAATTGTGGCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 16 |
| LEAP-Seq percent confirming: | 90.4255 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGCTTAGCACAGCCACA |
| Suggested primer 2: | ATCAATCTGCCCCTTGACAG |