| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069291 |
| Chromosome: | chromosome 9 |
| Location: | 6215744 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCACGGAAGGTAGCACGCCCAGCACTC |
| Internal bar code: | ATCTCGGGATCGCTGGACGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 377 |
| LEAP-Seq percent confirming: | 59.4787 |
| LEAP-Seq n confirming: | 251 |
| LEAP-Seq n nonconfirming: | 171 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCGTGTGGCTGTAGTAGT |
| Suggested primer 2: | ATCTGGACATTGCCTTCGAG |