| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.069292 |
| Chromosome: | chromosome 12 |
| Location: | 3390681 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496950 | (1 of 1) 3.4.13.5 - Xaa-methyl-His dipeptidase / X-methyl-His dipeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTGTGCTTAGTTGGATGTGACGTCACT |
| Internal bar code: | TCGAGAATACTCGGTGGTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 210 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTACAGCCGCAAAAGTG |
| Suggested primer 2: | ATGAGGCACTAGCAAGCGAT |