Insertion junction: LMJ.RY0402.069315_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.069315
Chromosome:chromosome 2
Location:2807224
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g094400 (1 of 133) IPR012336 - Thioredoxin-like fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGACGCAAGGGCGTCGGCGACAAGGAACC
Internal bar code:TATCCGGGCGTTGCGCATAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:1222
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGAAGATGTCCAGCGGAT
Suggested primer 2:CAGCGTTACATGGTTTGTGG