Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.069317 |
Chromosome: | chromosome_8 |
Location: | 3423218 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g374700 | FAP244 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCGAGGACGCCTTTGAGGGCTGCGAGCTGG |
Internal bar code: | CATGCCGACGAGTATAAAGCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 102 |
LEAP-Seq percent confirming: | 99.7041 |
LEAP-Seq n confirming: | 337 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCTTGATCTAGGGTCCCAC |
Suggested primer 2: | TACCGCTTTTCCAAACATCC |