| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069318 |
| Chromosome: | chromosome 14 |
| Location: | 3338375 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629960 | (1 of 2) 2.3.2.15 - Glutathione gamma-glutamylcysteinyltransferase / Phytochelatin synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACAGTTGCGGTCCCGCGACTGCGTGTCC |
| Internal bar code: | AGTCCCGTCAGGATCACCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 99.5475 |
| LEAP-Seq n confirming: | 220 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCCACTTCTCTGTACCAA |
| Suggested primer 2: | ACTCCAACAACGACACTCCC |