| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.069378 |
| Chromosome: | chromosome 9 |
| Location: | 1774857 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396350 | MITC3 | (1 of 2) PTHR24089//PTHR24089:SF51 - FAMILY NOT NAMED // MITOFERRIN-1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCTGGCCCTGCCTCCGCCTTTGCCTCC |
| Internal bar code: | CCGTACGATCTGACGTGAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 1243 |
| LEAP-Seq n nonconfirming: | 113 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCGGGAGCTCTGCTATAC |
| Suggested primer 2: | GGCGCATACCGTAGTTGAGT |