| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.069379 |
| Chromosome: | chromosome 6 |
| Location: | 8390737 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306850 | HEL31 | (1 of 1) K18655 - ATP-dependent RNA helicase DDX19/DBP5 [EC:3.6.4.13] (DDX19, DBP5); DEAD/DEAH box helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCGCAGTGAGTCTTGTCGGGTTGCTGA |
| Internal bar code: | AATCGTTTTATGTTGCGTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 99.8706 |
| LEAP-Seq n confirming: | 1544 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTGGCTATCTGTGGACGG |
| Suggested primer 2: | ACCACCACTTCCTTCATTGC |