Insertion junction: LMJ.RY0402.069382_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.069382
Chromosome:chromosome 1
Location:4116415
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g027050 (1 of 3) PF11527 - The ARF-like 2 binding protein BART (ARL2_Bind_BART) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTGCGGCCAGAACTTCGACATCTTCAAG
Internal bar code:GAAGGGGACCGGCGCGCGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:39
LEAP-Seq percent confirming:67.0022
LEAP-Seq n confirming:1198
LEAP-Seq n nonconfirming:590
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTCATTCTCCTGCTTGCC
Suggested primer 2:TCGCTAGGCATAGCAGGTTT