| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.069382 |
| Chromosome: | chromosome_1 |
| Location: | 4116415 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g027050 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACTGCGGCCAGAACTTCGACATCTTCAAG |
| Internal bar code: | GAAGGGGACCGGCGCGCGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 39 |
| LEAP-Seq percent confirming: | 67.0022 |
| LEAP-Seq n confirming: | 1198 |
| LEAP-Seq n nonconfirming: | 590 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCATTCTCCTGCTTGCC |
| Suggested primer 2: | TCGCTAGGCATAGCAGGTTT |