Insertion junction: LMJ.RY0402.069396_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.069396
Chromosome:chromosome 13
Location:851986
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g567363 FAP220 Flagellar Associated Protein 220; (1 of 14) PF02493 - MORN repeat (MORN) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGACACACGCGCCCGCCGCTGCTCGCTC
Internal bar code:CCGGTCACAAAGCGCGTGTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:48
LEAP-Seq percent confirming:99.8106
LEAP-Seq n confirming:6325
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCAACCCAAGGAGGAGTTTG
Suggested primer 2:CCTGAGTGCATCGCAAGTAA