| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.069449 |
| Chromosome: | chromosome 1 |
| Location: | 5302782 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037100 | (1 of 4) PTHR24347//PTHR24347:SF138 - SERINE/THREONINE-PROTEIN KINASE // PROTEIN F32D8.1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACTTGTATGGGTTGATGAATGCGATAA |
| Internal bar code: | GCCCACAGTCCGGGGTTGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 96.7903 |
| LEAP-Seq n confirming: | 9288 |
| LEAP-Seq n nonconfirming: | 308 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGTCGAGCTTCAGTCCC |
| Suggested primer 2: | AAGTACACGGAGGTGGATGC |