| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069450 |
| Chromosome: | chromosome 16 |
| Location: | 5897517 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677200 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCGATACCTGGATCTAGGAGAGCTGTT |
| Internal bar code: | CGGTGTGGACTTGCGCAGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1783 |
| LEAP-Seq percent confirming: | 97.9669 |
| LEAP-Seq n confirming: | 54787 |
| LEAP-Seq n nonconfirming: | 1137 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAACACGACCGTCAGCAA |
| Suggested primer 2: | AAGCGAAAGTCGACAAAGGA |