| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.069471 |
| Chromosome: | chromosome 12 |
| Location: | 5978693 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534800 | GCSP1,GCSP | (1 of 1) 1.4.4.2 - Glycine dehydrogenase (aminomethyl-transferring) / Glycine-cleavage complex P-protein; Glycine cleavage system, P protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGAGAGCAGGTTGAGTAGGGCCGGTCG |
| Internal bar code: | CCTCGGATCAGCTCGGCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 99.4813 |
| LEAP-Seq n confirming: | 959 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTCTGTGTGTGTGTGGG |
| Suggested primer 2: | GACAAGGCCAACAACATCCT |