| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069478 |
| Chromosome: | chromosome 13 |
| Location: | 3085837 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584700 | (1 of 2) PF02636 - Putative S-adenosyl-L-methionine-dependent methyltransferase (Methyltransf_28) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGTGCACCGTGCACCCATGCGTGCAGC |
| Internal bar code: | GTGGGTCGACGGTTCGAAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 99.6438 |
| LEAP-Seq n confirming: | 2238 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATCTCCAGGCACGTGTA |
| Suggested primer 2: | ATGGTGTTGTTGACAGCTCG |