| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.069487 |
| Chromosome: | chromosome 13 |
| Location: | 3474004 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587600 | (1 of 2) PTHR10907//PTHR10907:SF33 - REGUCALCIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCAACGATGCCCCGCAGTCATGCCCG |
| Internal bar code: | GTACCGCAGCAATGCGTGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 99.8032 |
| LEAP-Seq n confirming: | 7099 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAAGCCACTTAGACTTGC |
| Suggested primer 2: | CTTCTGCTGCTTCTCCGTGT |