| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.069504 |
| Chromosome: | chromosome_14 |
| Location: | 861537 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613400 | POC16 | Centriole proteome protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGGGGGGGGGAACAAGCCAGAAGCACGGG |
| Internal bar code: | AGGGAACTTCTTCGTGCGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 149 |
| LEAP-Seq percent confirming: | 63.1209 |
| LEAP-Seq n confirming: | 1258 |
| LEAP-Seq n nonconfirming: | 735 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTACGTGGTGGAAGACA |
| Suggested primer 2: | TTGGTGGTGGTGATAACCCT |