Insertion junction: LMJ.RY0402.069508_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.069508
Chromosome:chromosome 11
Location:2525307
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g475950 HLM16 putative N-methyltransferase; (1 of 5) IPR001214//IPR003616 - SET domain // Post-SET domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGCTGACCACTCCGTGCCGGATCTCTC
Internal bar code:AGGCACTTTCCGGAGGGGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:519
LEAP-Seq percent confirming:80.6212
LEAP-Seq n confirming:1739
LEAP-Seq n nonconfirming:418
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGGAACACGAACACACC
Suggested primer 2:GTTTCGTGTGTCGCTCTGAA