| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069543 |
| Chromosome: | chromosome 1 |
| Location: | 5377108 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037751 | (1 of 20) IPR013761 - Sterile alpha motif/pointed domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACACACCTACACTCCTGCAAATCATAC |
| Internal bar code: | GGGCCTCCACCAGTTGGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 99.7749 |
| LEAP-Seq n confirming: | 7091 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCGCGTAATTTTGAAACG |
| Suggested primer 2: | GCAAAGAGCGACATGATTGA |