| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069574 |
| Chromosome: | chromosome 2 |
| Location: | 2338180 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090750 | (1 of 1) PF08574 - Protein of unknown function (DUF1762) (DUF1762) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTACGACGTGTAGACCAACCTATTGCAG |
| Internal bar code: | GGAGGGTCCGCAGCGGGCCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.2155 |
| LEAP-Seq n confirming: | 2403 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGGACTGTCTGTGGTTT |
| Suggested primer 2: | GTTGGCGTAGTAGCTCTCGG |