| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.069598 |
| Chromosome: | chromosome 3 |
| Location: | 2292529 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158150 | (1 of 1) K12842 - U2-associated protein SR140 (SR140) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGATTGGAGACCGCGGGAAGGCCTGG |
| Internal bar code: | GTCGGGCTTGGTAGGCGGACGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 172 |
| LEAP-Seq percent confirming: | 84.375 |
| LEAP-Seq n confirming: | 378 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTAGCAGTGCAAATGA |
| Suggested primer 2: | CACCATACAATCCCCTCCAC |