| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.069636 |
| Chromosome: | chromosome 2 |
| Location: | 5194378 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105700 | (1 of 1) PF09837 - Uncharacterized protein conserved in bacteria (DUF2064) (DUF2064) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGCGTGCACCTCTGCCCCGCCCGTTAC |
| Internal bar code: | CTTGACGATGACGCTTCAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 98.7322 |
| LEAP-Seq n confirming: | 1246 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTCCCATGTTAGCCACT |
| Suggested primer 2: | GACAATGCAGAAGCTCCACA |