Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.069648 |
Chromosome: | chromosome_13 |
Location: | 4623412 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g604050 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGGCCGGGCAGCTCCCGCCATCTACCTCCC |
Internal bar code: | GACACCATAGCTGACCCACCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 419 |
LEAP-Seq percent confirming: | 90.2835 |
LEAP-Seq n confirming: | 6625 |
LEAP-Seq n nonconfirming: | 713 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTTGAGCAGGAAGAACAGC |
Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |