| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.069715 |
| Chromosome: | chromosome 3 |
| Location: | 4003573 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171950 | PPC2,PEPC2 | (1 of 2) K01595 - phosphoenolpyruvate carboxylase (ppc); Phosphoenolpyruvate carboxylase 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTTGGGGAGGAGGGGTAAGCAGACTGA |
| Internal bar code: | TTCCAATTGCCACGTACCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 32.0118 |
| LEAP-Seq n confirming: | 977 |
| LEAP-Seq n nonconfirming: | 2075 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACCGGCCCATTACTCAA |
| Suggested primer 2: | GAGGAAGAGGGAGAGGGAGA |