Insertion junction: LMJ.RY0402.069727_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.069727
Chromosome:chromosome 2
Location:2136492
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g089274 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTCAGAACAGCACCAGACACGTCGCCG
Internal bar code:CTCGTGGTTAAGCAGCTTAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:105
LEAP-Seq percent confirming:58.3486
LEAP-Seq n confirming:4148
LEAP-Seq n nonconfirming:2961
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGTGCCTCGTTTTCTC
Suggested primer 2:GCCGCTACAACCTCTGCTAC