| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.069743 |
| Chromosome: | chromosome 9 |
| Location: | 1778498 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396300 | PPX1,PPOX | (1 of 1) 1.3.3.4 - Protoporphyrinogen oxidase / Protoporphyrinogenase; Protoporphyrinogen oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAGGGTACAAGGAGGAGGTTGAGCAGG |
| Internal bar code: | GGGGTCCGTGTCGCCATAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 93.3837 |
| LEAP-Seq n confirming: | 494 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACACCCCTTGTTGTGTG |
| Suggested primer 2: | GGACCACACTGAGGGACAGT |