| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069754 |
| Chromosome: | chromosome 12 |
| Location: | 4780940 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524100 | ABH1,CBP80 | mRNA cap-binding protein; (1 of 1) K12882 - nuclear cap-binding protein subunit 1 (NCBP1, CBP80) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGCCCCGCTTCATCCATCCCTACCTGT |
| Internal bar code: | GCCGTTCGCACTCGGTCGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 57 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAGAACTCTGAACATGC |
| Suggested primer 2: | AGAGAAAGACGGTGCAAGGA |