| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069757 |
| Chromosome: | chromosome 1 |
| Location: | 7224130 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051950 | CND41,ASP2 | (1 of 2) 3.4.23.12 - Nepenthesin / Nepenthes aspartic proteinase; Pepsin-type aspartyl protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAACACTGCATACCGCAGCTGCTAGCAA |
| Internal bar code: | GCGCAAACGGGGTGGTGCCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 9 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 136 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTGGACAGGAACAGGTA |
| Suggested primer 2: | CCTCGCATTAGATAGCTGCC |