| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.069763 |
| Chromosome: | chromosome 10 |
| Location: | 3708921 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446250 | (1 of 1) K13339 - peroxin-6 (PEX6, PXAAA1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGGCGGGTGCAGTGGCAGGCGGTTCC |
| Internal bar code: | CGCAGTACGTACGCTTTAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 312 |
| LEAP-Seq percent confirming: | 99.4624 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTGAGAAAGCAAACGGA |
| Suggested primer 2: | AGGGTTGGGTGTGATAGCTG |