| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069780 |
| Chromosome: | chromosome 3 |
| Location: | 7340995 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206600 | AAD1 | Acetohydroxyacid dehydratase; (1 of 1) 4.2.1.9 - Dihydroxy-acid dehydratase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTGGGGAGTCAGAACTTTGCCATTTGCT |
| Internal bar code: | ATCCGGCCACGGAATCTGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 99.7044 |
| LEAP-Seq n confirming: | 1349 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGTGTGTGAAACGGCTT |
| Suggested primer 2: | CCAAAAGAGTTTCGTCGAGC |