| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069781 |
| Chromosome: | chromosome 3 |
| Location: | 5690407 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187700 | HEL19 | (1 of 1) PTHR24031//PTHR24031:SF252 - RNA HELICASE // SUBFAMILY NOT NAMED; DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACAGCCCCTCCGAGCGGCGCTCCATGG |
| Internal bar code: | GACACGCCCGACGCCCGGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 72.5178 |
| LEAP-Seq n confirming: | 17339 |
| LEAP-Seq n nonconfirming: | 6571 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | CAGCCCAAACTAAGCCAAAC |