| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069782 |
| Chromosome: | chromosome 16 |
| Location: | 5903184 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677150 | (1 of 2) 2.4.1.122 - Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACATACGGTAACGCAGCAACCGCAACAG |
| Internal bar code: | AAGCGGTCTTCGTCCGCGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 98.7042 |
| LEAP-Seq n confirming: | 6703 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTGTGGGGTTGTTCAAA |
| Suggested primer 2: | CACCGCTATCCTCATCCCTA |