| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069783 |
| Chromosome: | chromosome 12 |
| Location: | 8505388 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548000 | (1 of 1) K14550 - U3 small nucleolar RNA-associated protein 10 (UTP10, HEATR1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCTCCAGACCTGTGCGATAAAGCGACA |
| Internal bar code: | GCCGGCTATGGGAGCGAATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 95.0893 |
| LEAP-Seq n confirming: | 639 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGGAGCTGCTTGTCGTT |
| Suggested primer 2: | CTGTAGCTGTTCGCCATTCA |