Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.069785 |
Chromosome: | chromosome_2 |
Location: | 2809010 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g094450 | PHC21 | Cell wall protein pherophorin-C21 | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGTTGTTTATGGGGCAAGGCCGCCGGTTAC |
Internal bar code: | GACAGTGTTCCCGTCAGCACCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 437 |
LEAP-Seq percent confirming: | 99.9264 |
LEAP-Seq n confirming: | 1358 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGGGCTATGTGTACAGCCG |
Suggested primer 2: | AGGTTAAGGCGGGTAGCAAT |